Application of the ELISA and HPLC test for detection of aflatoxin in Pistachio

Scientific Research and Essays

Mehmet OZASLAN¹ 

关键词:  ;    Pistacia vera ;    L.;    aflatoxin;     ;    enzyme-linked immunosorbent assay.;   

DOI  :  10.5897/SRE10.707

学科分类：社会科学、人文和艺术（综合）

来源: Academic Journals

【 摘 要 】

Pistacia veraL. grows successfully in South-Eastern Anatolia due to its own ecological properties. Because of a high price ofP. veraL. in domestic and foreign markets it becomes a good source of income for the producers. The most faced problem in its exportation is ‘aflatoxin’. Several methods are used for the detection of aflatoxin inP. veraL. However, for these methods, the developed laboratories, experienced personals and high-cost are needed. In this study, aflatoxin content ofP. veraL. was investigated by using ELISA test which is used as a rapid, cheap and safe diagnostic technique in last decades. In addition, HPLC was applied for same samples and results obtained from ELISA were compared with those from HPLC. Plant materials used in this study were taken fromP. veraL. Depots and wholesale trade stores and consisted of 38 different sources ofP. veraL. samples. Aflatoxin B1 was determined over 2 ppb in 16 samples by ELISA. However there was no evidence about the total of aflatoxin. By HPLC, there were no evidence for aflatoxin B1 and also the total of aflatoxin. These results showed that the ELISA, is a molecular diagnostic test depends on protein content was more sensitive than HPLC. In this study ELISA test was used for the first time in the determination of alfatoxin content inP. veraL. kernels.

【 授权许可】

CC BY