Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice

Biological research: BR

Ramó¹  Luis F. Gonzá²  quez-Belmar⁴  rate⁴  Francisca Henrí⁵  Claudia Delgado-Acevedo⁶  Dennis L. Murphy⁶  lez⁸  Gloria Arriagada⁹  Pablo R. Moya^1,10  Marisol Cisternas-Olmedo^1,10  n Sotomayor-Zá^1,11 

[1] Laboratorio de NeurogenéLaboratorio de NeuroquíNúa y Plasticidad Cerebral, Instituto de Fisiología, Centro de Neurobiología, Facultad de Ciencias, Universidad de Valparaícleo Milenio Nu-MIND Biology of Neuropsychiatric Disorders, Valparaímica y Neurofarmacologíso, Chile;so, Valparaítica, Centro de Neurobiologí

关键词: EAAT3;    SLC1A1;    Neuronal glutamate transporter;    Obsessive–compulsive disorder;   

DOI  :  10.1186/s40659-017-0138-3

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Obsessive–compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1–3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus—brain areas that are relevant to OCD. Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice. Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.

【 授权许可】

CC BY