Cloning and heterologous expression of two cold-active lipases from the Antarctic bacterium Psychrobacter sp. G

Polar research

Shen Jihong¹  Cui Shuoshuo¹  Du Ning¹  Wang Shuai¹  Lin Xuezheng²  Xu Guoying³ 

[1] Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, SOA, 6 Xianxialing Road, Qingdao 266061, China;Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, SOA, 6 Xianxialing Road, Qingdao 266061, ChinaCorrespondence;Ocean College, Shandong University at Weihai, 180 Wenhuaxi Road, Weihai Shandong 264209, China

关键词: Antarctic;    cold-active lipase;    gene cloning;    gene expression;    Psychrobacter;   

DOI  :  10.3402/polar.v29i3.6087

学科分类：自然科学（综合）

来源: Co-Action Publishing

【 摘 要 】

Antarctic bacteria producing extracellular lipolytic enzymes with activity at low temperature were isolated, and the most promising strain, named G, was identified as a Psychrobacter species based on 16S rDNA sequence alignment. The genomic DNA of this bacterium was used to construct its plasmid genomic library into pUC118 plasmid vectors, and to screen the cold-active lipolytic enzyme genes. Two genes encoding for cold-active lipolytic enzymes, Lip-1452 (with an open reading frame of 1452 bp in length) and Lip-948 (with an open reading frame of 948 bp in length), were screened. The primary structure of the two lipases deduced from the nucleotide sequence showed a consensus pentapeptide containing the active serine (Lip-1452, GDSAG, and Lip-948, GNSMG) and a conserved His-Gly dipeptide in the N-terminal part of the enzyme. Protein sequence alignment and conserved regions analysis indicated that the two lipases probably belonged to family IV and family V of the bacterial lipolytic enzymes, respectively. Th...

【 授权许可】

CC BY-NC