Viral Retasking of hBre1/RNF20 to Recruit hPaf1 for Transcriptional Activation

PLoS Pathogens

Michael J. Cohen¹  Gregory J. Fonseca¹  John W. Barrett²  Anthony C. Nichols³  Joe S. Mymryk³ 

[1] Department of Microbiology & Immunology, University of Western Ontario, London, Ontario, Canada;Department of Oncology, University of Western Ontario, London, Ontario, Canada;Department of Otolaryngology-Head and Neck Surgery, University of Western Ontario, London, Ontario, Canada

关键词: DNA transcription;    Small interfering RNAs;    Gene expression;    Immunoprecipitation;    Transcriptional control;    Viral gene expression;    DNA-binding proteins;    Luciferase;   

DOI  :  10.1371/journal.ppat.1003411

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Upon infection, human adenovirus (HAdV) must activate the expression of its early genes to reprogram the cellular environment to support virus replication. This activation is orchestrated in large part by the first HAdV gene expressed during infection, early region 1A (E1A). E1A binds and appropriates components of the cellular transcriptional machinery to modulate cellular gene transcription and activate viral early genes transcription. Previously, we identified hBre1/RNF20 as a target for E1A. The interaction between E1A and hBre1 antagonizes the innate antiviral response by blocking H2B monoubiquitination, a chromatin modification necessary for the interferon (IFN) response. Here, we describe a second distinct role for the interaction of E1A with hBre1 in transcriptional activation of HAdV early genes. Furthermore, we show that E1A changes the function of hBre1 from a ubiquitin ligase involved in substrate selection to a scaffold which recruits hPaf1 as a means to stimulate transcription and transcription-coupled histone modifications. By using hBre1 to recruit hPaf1, E1A is able to optimally activate viral early transcription and begin the cycle of viral replication. The ability of E1A to target hBre1 to simultaneously repress cellular IFN dependent transcription while activating viral transcription, represents an elegant example of the incredible economy of action accomplished by a viral regulatory protein through a single protein interaction.

【 授权许可】

CC BY