PLoS Pathogens | |
ROP18 Is a Rhoptry Kinase Controlling the Intracellular Proliferation of Toxoplasma gondii | |
Jean François Dubremetz1  Stefan T Arold1  Henri Vial2  Hiba El Hajj3  Gilles Labesse3  Maryse Lebrun3  | |
[1] Centre de Biochimie Structurale, Centre National de la Recherche Scientifique, Université Montpellier 1, Montpellier, France;Centre de Biochimie Structurale, Institut National de la Santé et de la Recherche Médicale, Montpellier, France;UMR 5539, Centre National de la Recherche Scientifique, Université de Montpellier 2, Montpellier, France | |
关键词: Parasitic diseases; Tachyzoites; Sequence motif analysis; Host cells; Toxoplasma gondii; Recombinant proteins; Membrane proteins; Parasite replication; | |
DOI : 10.1371/journal.ppat.0030014 | |
学科分类:生物科学(综合) | |
来源: Public Library of Science | |
【 摘 要 】
Toxoplasma gondii is an obligate intracellular parasite for which the discharge of apical organelles named rhoptries is a key event in host cell invasion. Among rhoptry proteins, ROP2, which is the prototype of a large protein family, is translocated in the parasitophorous vacuole membrane during invasion. The ROP2 family members are related to protein-kinases, but only some of them are predicted to be catalytically active, and none of the latter has been characterized so far. We show here that ROP18, a member of the ROP2 family, is located in the rhoptries and re-localises at the parasitophorous vacuole membrane during invasion. We demonstrate that a recombinant ROP18 catalytic domain (amino acids 243–539) possesses a protein-kinase activity and phosphorylate parasitic substrates, especially a 70-kDa protein of tachyzoites. Furthermore, we show that overexpression of ROP18 in transgenic parasites causes a dramatic increase in intra-vacuolar parasite multiplication rate, which is correlated with kinase activity. Therefore, we demonstrate, to our knowledge for the first time, that rhoptries can discharge active protein-kinases upon host cell invasion, which can exert a long-lasting effect on intracellular parasite development and virulence.
【 授权许可】
CC BY
【 预 览 】
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RO201902010944168ZK.pdf | 748KB | download |