Quantitative Proteomics of Intracellular Campylobacter jejuni Reveals Metabolic Reprogramming

PLoS Pathogens

Xiaoyun Liu¹ 

[1] Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, United States of America

关键词: Biosynthesis;    Respiratory infections;    Intracellular pathogens;    Metabolic pathways;    Oxygen;    Proteomes;    Campylobacter jejuni;    Protein metabolism;   

DOI  :  10.1371/journal.ppat.1002562

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Campylobacter jejuni is the major cause of bacterial food-borne illness in the USA and Europe. An important virulence attribute of this bacterial pathogen is its ability to enter and survive within host cells. Here we show through a quantitative proteomic analysis that upon entry into host cells, C. jejuni undergoes a significant metabolic downshift. Furthermore, our results indicate that intracellular C. jejuni reprograms its respiration, favoring the respiration of fumarate. These results explain the poor ability of C. jejuni obtained from infected cells to grow under standard laboratory conditions and provide the bases for the development of novel anti microbial strategies that would target relevant metabolic pathways.

【 授权许可】

CC BY