Host Imprints on Bacterial Genomes—Rapid, Divergent Evolution in Individual Patients

PLoS Pathogens

Jenny Grönberg-Hernandez¹  Bryndis Ragnarsdottir¹  Catharina Svanborg¹  Eliora Z. Ron²  Dvora Biran²  Björn Wullt³  Jörg Hacker⁴  Rolf Daniel⁵  Gerhard Gottschalk⁵  Heiko Liesegang⁵  Elzbieta Brzuszkiewicz⁵  Michael Hecker⁶  Birgit Voigt⁶  Jaroslaw Zdziarski⁷  Ulrich Dobrindt⁸ 

[1] Department of Microbiology, Immunology and Glycobiology, Institute of Laboratory Medicine, Lund University, Lund, Sweden;Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel;Department of Urology, Lund University Hospital, Lund, Sweden;German Academy of Sciences Leopoldina, Halle/Saale, Germany;Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany;Institute for Microbiology, Ernst-Moritz-Arndt-University Greifswald, Greifswald, Germany;Institute for Molecular Biology of Infectious Diseases, Julius-Maximilians-University Würzburg, Würzburg, Germany;Singapore Immunology Network (SIgN), Biomedical Sciences Institutes, Agency for Science, Technology, and Research (A*STAR), Singapore, Singapore

关键词: Bacterial genomics;    Urine;    Genomic imprinting;    Regulator genes;    Point mutation;    Virulence factors;    Bladder;    Comparative genomics;   

DOI  :  10.1371/journal.ppat.1001078

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Bacteria lose or gain genetic material and through selection, new variants become fixed in the population. Here we provide the first, genome-wide example of a single bacterial strain's evolution in different deliberately colonized patients and the surprising insight that hosts appear to personalize their microflora. By first obtaining the complete genome sequence of the prototype asymptomatic bacteriuria strain E. coli 83972 and then resequencing its descendants after therapeutic bladder colonization of different patients, we identified 34 mutations, which affected metabolic and virulence-related genes. Further transcriptome and proteome analysis proved that these genome changes altered bacterial gene expression resulting in unique adaptation patterns in each patient. Our results provide evidence that, in addition to stochastic events, adaptive bacterial evolution is driven by individual host environments. Ongoing loss of gene function supports the hypothesis that evolution towards commensalism rather than virulence is favored during asymptomatic bladder colonization.

【 授权许可】

CC BY