Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation

Polar research

Søren J. Sørensen¹  Waleed Abu Al-Soud¹  Annette K. Møller²  Ditte A. Søborg²  Niels Kroer³ 

[1] Department of BiologyUniversity of Copenhagen, Sølvgade 83H, DK-1307 K Copenhagen, Denmark;Department of Environmental ScienceAarhus University, Frederiksborgvej 399, DK-4000 Roskilde, Denmark;Department of Environmental ScienceAarhus University, Frederiksborgvej 399, DK-4000 Roskilde, DenmarkCorrespondence

关键词: Taxonomic diversity;    microbial assemblages;    bacterial density;    DOC;   

DOI  :  10.3402/polar.v32i0.17390

学科分类：自然科学（综合）

来源: Co-Action Publishing

【 摘 要 】

The bacterial community structures in High-Arctic snow over sea ice and an ice-covered freshwater lake were examined by pyrosequencing of 16S rRNA genes and 16S rRNA gene sequencing of cultivated isolates. Both the pyrosequence and cultivation data indicated that the phylogenetic composition of the microbial assemblages was different within the snow layers and between snow and freshwater. The highest diversity was seen in snow. In the middle and top snow layers, Proteobacteria, Bacteroidetes and Cyanobacteria dominated, although Actinobacteria and Firmicutes were relatively abundant also. High numbers of chloroplasts were also observed. In the deepest snow layer, large percentages of Firmicutes and Fusobacteria were seen. In freshwater, Bacteroidetes, Actinobacteria and Verrucomicrobia were the most abundant phyla while relatively few Proteobacteria and Cyanobacteria were present. Possibly, light intensity controlled the distribution of the Cyanobacteria and algae in the snow while carbon and nitrogen fix...

【 授权许可】

CC BY-NC