Impact of Mycobacterium ulcerans Biofilm on Transmissibility to Ecological Niches and Buruli Ulcer Pathogenesis

PLoS Pathogens

Mary Jackson¹  Jana Korduláková¹  Stewart T Cole²  Priscille Brodin²  Gilles Reysset²  Jean-Paul Saint André³  Jane Cottin⁴  Geneviève Milon⁵  Marie Laure Joly Guillou⁶  Jacques Aubry⁷  Laurent Marsollier⁷  Etienne Carbonnelle⁸  Petra Tafelmeyer⁸  Pierre Legras⁹  Céline Leroy^1,10 

[1] Equipe Avenir Inserm, Biology of Intracellular Pathogens, Institut Pasteur Korea, Seoul, South Korea;Groupe d'Etude des Interactions Hôtes Parasites et Animalerie Hospitalo-Universitaire, Université d'Angers, Angers, France;Inserm U570, Paris, France;Inserm U601, Nantes, France;Plate Forme 3-Protéomique, Insitut Pasteur, Paris, France;Unité d'Immunophysiologie et Parasitisme Intracellulaire, Institut Pasteur, Paris, France;Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France;Unité de Génétique Mycobactérienne, Insitut Pasteur, Paris, France;Université René Descartes, Paris, France;Université de Nantes, Nantes, France

关键词: Mycobacterium ulcerans;    Vesicles;    Bacterial biofilms;    Extracellular matrix;    Carbohydrates;    Bacteria;    Buruli ulcer;    Lipids;   

DOI  :  10.1371/journal.ppat.0030062

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The role of biofilms in the pathogenesis of mycobacterial diseases remains largely unknown. Mycobacterium ulcerans, the etiological agent of Buruli ulcer, a disfiguring disease in humans, adopts a biofilm-like structure in vitro and in vivo, displaying an abundant extracellular matrix (ECM) that harbors vesicles. The composition and structure of the ECM differs from that of the classical matrix found in other bacterial biofilms. More than 80 proteins are present within this extracellular compartment and appear to be involved in stress responses, respiration, and intermediary metabolism. In addition to a large amount of carbohydrates and lipids, ECM is the reservoir of the polyketide toxin mycolactone, the sole virulence factor of M. ulcerans identified to date, and purified vesicles extracted from ECM are highly cytotoxic. ECM confers to the mycobacterium increased resistance to antimicrobial agents, and enhances colonization of insect vectors and mammalian hosts. The results of this study support a model whereby biofilm changes confer selective advantages to M. ulcerans in colonizing various ecological niches successfully, with repercussions for Buruli ulcer pathogenesis.

【 授权许可】

CC BY