Unconventional Repertoire Profile Is Imprinted during Acute Chikungunya Infection for Natural Killer Cells Polarization toward Cytotoxicity

PLoS Pathogens

Caroline Petitdemange¹  Patrice Debré¹  Vivien Béziat²  Vincent Vieillard³  Nadia Wauquier³  Pierre Becquart⁴  Eric M. Leroy⁴ 

[1] INSERM UMR-S 945, Immunité et Infection, Hôpital Pitié-Salpêtrière, Paris, France;UMR 224 IRD/CNRS/UM1, Montpellier, France;Unité des Maladies Virales Emergentes, Centre International de Recherches Médicales de Franceville, Franceville, Gabon;Université Pierre et Marie Curie, Paris, France

关键词: NK cells;    Chikungunya virus;    Chikungunya infection;    Viral load;    Flow cytometry;    Immune response;    Cell degranulation;    Arboviral infections;   

DOI  :  10.1371/journal.ppat.1002268

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Chikungunya virus (CHIKV) is a worldwide emerging pathogen. In humans it causes a syndrome characterized by high fever, polyarthritis, and in some cases lethal encephalitis. Growing evidence indicates that the innate immune response plays a role in controlling CHIKV infection. We show here that CHIKV induces major but transient modifications in NK-cell phenotype and function soon after the onset of acute infection. We report a transient clonal expansion of NK cells that coexpress CD94/NKG2C and inhibitory receptors for HLA-C1 alleles and are correlated with the viral load. Functional tests reveal cytolytic capacity driven by NK cells in the absence of exogenous signals and severely impaired IFN-γ production. Collectively these data provide insight into the role of this unique subset of NK cells in controlling CHIKV infection by subset-specific expansion in response to acute infection, followed by a contraction phase after viral clearance.

【 授权许可】

CC BY