Bacteria-Induced Dscam Isoforms of the Crustacean, Pacifastacus leniusculus

PLoS Pathogens

Pikul Jiravanichpaisal¹  Irene Söderhäll¹  Apiruck Watthanasurorot¹  Haipeng Liu²  Kenneth Söderhäll³ 

[1] Department of Comparative Physiology, Uppsala University, Uppsala, Sweden;National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, Thailand;State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, Xiamen, Fujian, People's Republic of China

关键词: Crayfish;    Staphylococcus aureus;    Hemocytes;    Recombinant proteins;    Phagocytosis;    Sequence motif analysis;    Polymerase chain reaction;    Antibodies;   

DOI  :  10.1371/journal.ppat.1002062

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The Down syndrome cell adhesion molecule, also known as Dscam, is a member of the immunoglobulin super family. Dscam plays an essential function in neuronal wiring and appears to be involved in innate immune reactions in insects. The deduced amino acid sequence of Dscam in the crustacean Pacifastacus leniusculus (PlDscam), encodes 9(Ig)-4(FNIII)-(Ig)-2(FNIII)-TM and it has variable regions in the N-terminal half of Ig2 and Ig3 and the complete Ig7 and in the transmembrane domain. The cytoplasmic tail can generate multiple isoforms. PlDscam can generate more than 22,000 different unique isoforms. Bacteria and LPS injection enhanced the expression of PlDscam, but no response in expression occurred after a white spot syndrome virus (WSSV) infection or injection with peptidoglycans. Furthermore, PlDscam silencing did not have any effect on the replication of the WSSV. Bacterial specific isoforms of PlDscam were shown to have a specific binding property to each tested bacteria, E. coli or S. aureus. The bacteria specific isoforms of PlDscam were shown to be associated with bacterial clearance and phagocytosis in crayfish.

【 授权许可】

CC BY