Interaction of Polymerase Subunit PB2 and NP with Importin α1 Is a Determinant of Host Range of Influenza A Virus

PLoS Pathogens

Hans-Dieter Klenk¹  Astrid Herwig¹  Gülsah Gabriel¹ 

[1] Institute of Virology, Philipps University Marburg, Germany

关键词: Polymerases;    Birds;    Cell binding;    Influenza A virus;    Viral replication;    Cytoplasm;    Microbial mutation;    293T cells;   

DOI  :  10.1371/journal.ppat.0040011

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

We have previously reported that mutations in the polymerase proteins PB1, PB2, PA, and the nucleocapsid protein NP resulting in enhanced transcription and replication activities in mammalian cells are responsible for the conversion of the avian influenza virus SC35 (H7N7) into the mouse-adapted variant SC35M. We show now that adaptive mutations D701N in PB2 and N319K in NP enhance binding of these proteins to importin α1 in mammalian cells. Enhanced binding was paralleled by transient nuclear accumulation and cytoplasmic depletion of importin α1 as well as increased transport of PB2 and NP into the nucleus of mammalian cells. In avian cells, enhancement of importin α1 binding and increased nuclear transport were not observed. These findings demonstrate that adaptation of the viral polymerase to the nuclear import machinery plays an important role in interspecies transmission of influenza virus.

【 授权许可】

CC BY