Separation and purification of three stilbenes from the radix of Polygonum cillinerve (Nakai) Ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography

Quimica nova

Xing, Yuxiu¹  Chi, Xiaofeng¹  Dong, Qi¹  Hu, Fengzu¹  Xiao, Yuancan¹ 

[1] Northwest Institute of Plateau Biology, P. R. China

关键词: Polygonum cillinerve (Nakai) Ohwl;    HSCCC;    stilbenes.;   

DOI  :  10.5935/0100-4042.20140256

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

An effective method for the rapid separation and purification of three stilbenes from the radix of Polygonum cillinerve (Nakai) Ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was successfully established. In the present study, a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:1:4:2, v/v/v/v) was used for HSCCC separation. A one-step separation in 4 h from 150 mg of crude extract produced 26.3 mg of trans-resveratrol-3-O-glucoside, 42.0 mg of pieceid-2"-O-gallate, and 17.9 mg of trans-resveratrol with purities of 99.1%, 97.8%, and 99.4%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these compounds were identified by nuclear magnetic resonance (NMR) spectroscopy.

【 授权许可】

CC BY