The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: human fetal liver;    B cell development;   

DOI  :  10.1189/jlb.0113048

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

CD27,amemberoftheTNFRsuperfamily,isusedtoidentifyhumanmemoryBcells.Nonetheless,CD27+BcellsarepresentinpatientswithHIGM1syndromewhoareunabletogenerateGCsormemoryBcells.CD27+IgD+fetalBcellsarepresentinumbilicalcordblood,andCD27mayalsobeamarkerofthehumanB1‐likeBcells.TodefinetheoriginofnaïveCD27+IgD+humanBcells,westudiedBcelldevelopmentinbothfetalandadulttissues.InhumanFL,mostCD19+cellscoexpressedCD10,amarkerofhumandevelopingBcells.SomeCD19+CD10+BcellsexpressedCD27,andthesefetalCD27+cellswerepresentinthepro‐B,pre‐B,andimmature/transitionalBcellcompartments.LowerfrequenciesofphenotypicallyidenticalcellswerealsoidentifiedinadultBM.CD27+pro‐B,pre‐B,andimmature/transitionalBcellsexpressedrecombinationactivatinggene‐1,terminaldeoxynucleotidyltransferaseandVpre‐BmRNAcomparablytotheirCD27−counterparts.CD27+andCD27−developingBcellsshowedsimilarIgheavychaingeneusagewithlowlevelsofmutations,suggestingthatCD27+developingBcellsaredistinctfrommutatedmemoryBcells.Despitethesesimilarities,CD27+developingBcellsdifferedfromCD27−developingBcellsbytheirincreasedexpressionofLIN28B,atranscriptionfactorassociatedwiththefetallymphoidlineagesofmice.Furthermore,CD27+pro‐BcellsefficientlygeneratedIgM+IgD+immature/transitionalBcellsinvitro.OurobservationssuggestthatCD27expressionduringBcelldevelopmentidentifiesaphysiologicstateorlineageforhumanBcelldevelopmentdistinctfromthememoryBcellcompartment...

【 授权许可】

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