Age and low levels of circulating vitamin D are associated with impaired innate immune function

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: aging;    cathelicidin;    innate immunity;    Toll‐;    like receptor;    vitamin D;   

DOI  :  10.1189/jlb.1011523

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

ThisstudyinvestigatedinvivotheinfluenceofageandvitaminDstatusoninnateimmunefunctioninHC.Serum25OHDwasmeasuredin71HC.TLRexpressiononvarioussubpopulationsofPBMCs,aswellasTLRfunctionbystimulatingPBMCswithspecificligands,wasassessedbyflowcytometry.CirculatingcathelicidinlevelsweredeterminedbyELISA.Serum25OHDlevelsdecreasedwithage,andtherewasasignificantinversecorrelationbetween25OHDlevelsandage.Therewasanegativecorrelationbetweenserum25OHDlevelsandMFIexpressionofTLR7onBcells,Tcells,andmonocytes.TLR7function,addressedbyinvitrostimulationwithaspecificagonist,wassignificantlycorrelatedwithserum25OHDlevels,andthiswasespeciallyaresultoftheresultsinHColderthan60years.MFIexpressionofTLR5onTcellsandTLR2onmonocyteswasalsonegativelycorrelatedwithserum25OHDlevels.TLR1(monocytes)andTLR2(monocytes)expressionwaspositivelycorrelatedwithage.Furthermore,TLR4andTLR8functionwasnegativelycorrelatedwithage.Circulatingcathelicidinlevelsdecreasedwithageandwerepositivelycorrelatedwith25OHDlevels.AgingisaccompaniedbychangesinexpressionandfunctionofseveralTLRs.Serum25OHDlevelsdecreasewithageandarealsoassociatedwithachangeinexpressionanddefectivefunctionofcertainTLRs,especiallythoseinvolvedinviralresponse...

【 授权许可】

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