Protective effect of histamine microinjected into the cerebellar fastigial nucleus on stress-induced gastric mucosal damage in rats

American Journal of Translational Research

Xiao Qiao¹ 

关键词: Histamine;    fastigial nucleus;    lateral hypothalamic area;    stress-induced gastric mucosal damage;    decussation of superior cerebellar peduncle;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Aims: We investigated the effffects and the possible mechanism of microinjection of histamine into cerebellar fastigial nucleus (FN) on stress-induced gastric mucosal damage (SGMD) in rats. The effect of microinjection of histamine into FN on SGMD was observed. Methods: The model of SGMD was established by restraint and water (21 ± 1°C)-immersion (RWI) for 3 h in rats. The gastric mucosal damage index indicated the severity of SGMD. Western blotting was performed to assess gastric mucosal cell apoptosis and proliferation. Results: We observed that histamine microinjection into the FN markedly attenuated SGMD in a dose-dependent manner, and was prevented by pre-treatment with the ranitidine (a selective histamine H2 receptor antagonist) into the FN. The effect of histamine was abolished by pre-treatment with 3-MPA (a glutamic acid decarboxylase antagonist) into the FN. There was a decrease in the discharge frequency of greater splanchnic nerve, and an increase in gastric mucosal blood flow after histamine injection into the FN. Additionally, anti-apoptotic and anti-oxidative factors of gastric mucosa might be involved in this process. Conclusion: The exogenous histamine in FN participates in the regulation of SGMD, and our results may help to provide new ideas on the treatment of gastroenterological diseases.

【 授权许可】

CC BY-NC