MiR-378 Promotes the Migration of Liver Cancer Cells by Down-Regulating Fus Expression

Cellular Physiology and Biochemistry

关键词: MicroRNA;    Liver cancer cell;    Migration;    Invasion;    Metastasis;   

DOI  :  10.1159/000369669

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background miR-378 regulates osteoblast differentiation and participates in tumor cell self-renewal and chemo-resistance. However, the function of miR-378 in liver cancer cell migration has not been reported to date. Methods miR-378 expression was examined using real-time quantitative PCR. HepG2 cell migration and liver cell invasion were examined using wound-healing and cell invasion assays. Additionally, HepG2 cell metastasis was analyzed in nude mice. Results miR-378 over-expression enhances HepG2 cell proliferation, migration and liver cell invasion. Typical metastatic lesions were found in the livers of mice injected with miR-378-transfected cells, and high levels of the CMV promoter were detected in the nodules, indicating that miR-378 promoted the metastasis of the tumor cells to the liver. We also demonstrated that miR-378 down-regulated Fus expression. Conclusions Our results suggested that miR-378 enhanced cell migration and metastasis by down-regulating Fus expression.

【 授权许可】

CC BY-NC-ND