New insights on the natural killer cell repertoire from a thorough analysis of cord blood cells

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: NK cells;    KIR;    NKG2C;    differentiation;   

DOI  :  10.1189/jlb.1HI0116-036R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

AlthoughCBNKcellsarecharacterizedasimmaturelymphocytes,theirimpressiveexpansionandefficientgraft‐versus‐leukemiaresponsehavebeenhighlightedearlyafterUCBT.TobetterevaluatetheirpotentialassourceofeffectiveNKcells,werevisitedthestudyofNKcellrepertoirefromalargecohortofCBsamples.OurstudyshowedthattheCBNKcellrepertoireappearstobeconstructedearly,dependingonKIRgenecontent,butnotontheautologousHLAenvironment.NKG2AwasexpressedonalargeproportionofCBNKcellsthatinverselycorrelatedwithKIR+NKcellfrequency.Self‐HLAclassImolecule–educatedCBKIR+NKcellspresentalowerspontaneouslysisthandotheiradultcounterparts,whichisprobablyrelatedtothelowexpressionofactivatingNKreceptors.WedescribeforthefirsttimeaproliferativeandcytotoxicNKG2C+NKcellsubsetrepresentingmorethan10%ofCBNKcells.NKG2AstronglyinhibitedCBNKcelldegranulation,anditscoexpressiononNKG2C+NKcellsmaycontributetolimitingtheiractivation.Overall,theCBNKcellrepertoireisconstructedearlyandharborsnumerousfunctionalabilitiessharedbyadultNKcells.Inaddition,theirnaïveviralstatusandfastexpansionconfernumerousadvantagesinimmunotherapyonCBNKcells...

【 授权许可】

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