| Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
| Regulation of neutrophil‐mediated killing of Staphylococcus aureus and chemotaxis by c‐jun NH2 terminal kinase | |
| 关键词: JNK; TAT‐; JIP peptide; superoxide production; phagocytosis; adherence; mechanism of bactericidal activity; | |
| DOI : 10.1189/jlb.0609399 | |
| 学科分类：生理学 | |
| 来源: Federation of American Societies for Experimental Biology | |
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【 摘 要 】
TheroleofJNKinneutrophilchemotaxisandkillingofmicrobialpathogensremainsunclear.Usingarecentlydescribedcell‐permeablepeptideinhibitoroftheJNKpathway,basedontheJBDofJIP‐1,coupledtotheproteintransductiondomainofHIV‐TAT(TAT‐JIP),inassociationwithcontrolpeptides,wedemonstratethattheJNKpathwayplaysamajorroleinregulatinghumanneutrophilchemotaxisandkillingofmicrobialpathogens.Serum‐opsonizedStaphylococcusaureuselicitedJNKactivationandc‐junphosphorylation.TheactivationoftheJNKpathwayandbactericidalactivitywereinhibitedbytheTAT‐JIPpeptide.ThestimulationofoxygenradicalgenerationbyS.aureuswasdependentontheJNKsignalingpathway,aswasthephagocytosisofserum‐opsonizedbacteria.ChemotaxistoactivatedserumcomplementbutnotrandommigrationwasinhibitedbytheTAT‐JIPpeptide.ThefindingsdemonstrateamajorrolefortheJNKsignalingpathwayinneutrophil‐mediateddefenseagainstmicrobialpathogens...
【 授权许可】
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【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201901236933318ZK.pdf | 917KB |  download |
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