期刊论文详细信息
Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society
The increased expression of IL‐23 in inflammatory bowel disease promotes intraepithelial and lamina propria lymphocyte inflammatory responses and cytotoxicity
关键词: Crohnˈ;    s disease;    mononuclear cell;    ulcerative colitis;   
DOI  :  10.1189/jlb.0810456
学科分类:生理学
来源: Federation of American Societies for Experimental Biology
PDF
【 摘 要 】

ThisstudyanalyzedIL‐23p19expressionininflamedmucosaofIBDandtheroleintheinductionofIELandNKcellactivationaswellasTh17celldifferentiation.ExpressionofIL‐23p19wasperformedbyimmunohistochemistryandquantitativereal‐timePCR.ExpressionofIL‐23Rwasassessedbyflowcytometry.CytolyticactivitiesofIELandNKcellsbyIL‐23weredeterminedbyastandard51Cr‐releaseassay.CytokinelevelswereanalyzedbyELISAandquantitativereal‐timePCR.ExpressionofIL‐23p19wasincreasedsignificantlyininflamedmucosaofCDcomparedwiththatinUCandhealthycontrols.Double‐stainingconfirmedthatIL‐23p19+cellsweremainlyCD68+macrophages/DCs.IL‐23R+cellswereincreasedsignificantlyinPB‐andLP‐CD4+and‐CD8+TandNKcells.IL‐23markedlypromotedIBDIELandNKcellactivationandcytotoxicityandtriggeredIBDPB‐andLP‐TcellstosecretesignificantlyhigherlevelsofIFN‐γ,TNF,IL‐2,andIL‐17Acomparedwithcontrols.Importantly,IL‐23promotedIBDPB‐orLP‐CD4+TcellstodifferentiateintoTh17cells,characterizedbyincreasedexpressionofIL‐17AandRORC.Anti‐TNFtreatmentcouldmarkedlyreduceIL‐23expressionandTh17cellinfiltrationininflamedmucosaofCDpatients.ThesedataindicatethatIL‐23ishighlyexpressedininflamedmucosaofIBDandplaysanimportantroleintheinductionofIEL,NK,andTcellactivation,proinflammatorycytokinesecretion,andTh17celldifferentiation.TargetedtherapydirectedagainstIL‐23p19mayhaveatherapeuticroleintreatmentofIBD...

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO201901236870578ZK.pdf 2258KB PDF download
  文献评价指标  
  下载次数:6次 浏览次数:13次