Tmem176B and Tmem176A are associated with the immature state of dendritic cells

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: cell activation;    antigen presentation;   

DOI  :  10.1189/jlb.1109738

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

DCsplayacentralroleinthedevelopmentofinnateandadaptiveimmunitybutalsointheinductionandmaintenanceofimmunetolerance.IdentificationoffactorsthatgovernDCactivation,theirmaturationstate,andtheircapacitytoinduceproinflammatoryortolerogeneicresponsesthereforerepresentsacrucialaimofresearch.Wepreviouslyidentifiedanewmolecule,Tmem176B(whichwenamedTORIDinitially),ashighlyexpressedinamodelofallografttoleranceintherat.WeshowedthatitsoverexpressioninratDCsblockedtheirmaturation,suggestingaroleforthismoleculeinthematurationprocess.TocharacterizethefunctionofTmem176Bfurther,weusedasplit‐ubiquitinyeast,two‐hybridsystemtoidentifyinteractingpartnersandfoundthatTmem176BassociatedwithitselfbutalsowithTmem176A,amembraneproteinsimilartoTmem176B.Interestingly,thesetwomoleculesshowedsimilarmRNAexpressionpatternsamongvariousmurinetissuesandimmunecellsandwerebothdown‐regulatedfollowingDCmaturation.Inaddition,weshowedthatinusingRNAi,thesemoleculesarebothinvolvedinthemaintenanceoftheimmaturestateoftheDCs.Takentogether,thesedatasuggestthatTmem176BandTmem176AassociatetoformmultimersandrestrainDCmaturation.Therefore,thesetwomoleculesmayrepresentvalidtargetstoregulateDCfunction...

【 授权许可】

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