Down-Regulation of MiR-30c Promotes the Invasion of Non-Small Cell Lung Cancer by Targeting MTA1

Cellular Physiology and Biochemistry

关键词: MicroRNA-30c;    MTA1;    Invasion;    NSCLC;   

DOI  :  10.1159/000354452

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background The connection between microRNA expression and lung cancer development has been identified in recent literature. However, the mechanism of microRNA has been poorly elucidated in non-small-cell lung cancer (NSCLC). Methods and Results Comparing with adjacent tissues (n=75), miR-30c has a lower expression in lung cancer specimens (n=75). The knockdown of miR-30c enhanced the invasion of A549 cells; meanwhile, the overexpression of miR-30c could reverse the effect of the knockdown of miR-30c in vitro. A luciferase assay revealed that miR-30c was directly bound to the 3‘-untranslated regions (3‘-UTR) of MTA1. QRT-PCR and western blot shows MTA1 was up-regulated in mRNA and protein levels. The effect taken on the invasion of NSCLC by overexpression of MTA1 works the same as down-regulated miR-30c. Conclusion miR-30c may play a pivotal role in controlling lung cancer invasion through regulating MTA1in NSCLC.

【 授权许可】

CC BY-NC-ND