Recombinant human mannose‐binding lectin dampens human alveolar macrophage inflammatory responses to influenza A virus in vitro

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: innate immunity;    C‐;    type lecin;    TNF;    respiratory burst;    apoptosis;    biological response modifier;   

DOI  :  10.1189/jlb.0313161

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

IAVpneumoniaremainsaseriousglobalhealthproblem,andpreventativeandtherapeuticstrategiesremainlimited.AMarecriticaleffectorcellsinthecontrolofinfluenza,impairingIAVreplication,promotingIAVclearance,andpromotingefferocytosisandresolutionoflunginflammation.MBL,aninnateimmunepatternrecognitionmolecule,presentinthelungs,bindsIAV,andplasmaMBLdeficiencyisassociatedwithincreasedsusceptibilitytoIAV,althoughthemechanismremainsincompletelyunderstood,andtheinfluenceofMBLontheIAV‐AMinteractionhasnotbeenestablished.Inthecurrentstudy,focusingonhumanmacrophages(U937celllineandclinicallyrelevanthumanAM),datademonstratedthatunopsonizedIAVisreadilyinternalized,inducedreleaseofTNFandROS,andpromotedmacrophageapoptosis.Incontrast,IAV,opsonizedwithrhMBL,reducedIAVuptakeandmacrophageapoptosisanddramaticallyreducedTNFreleaseandROS.Macrophagehost‐defenseresponseswerereducedfurtherinthepresenceofMASPs.Takentogether,thesedatasupporttheconceptthatrhMBLmayserveaprotectiveinnatehostresponseandacriticalbiologicalresponsemodifierfunctionbylimitingAMinflammation,oxidativeinjury,andAMapoptosis,whichmayalloweffectiveIAVclearancewhilelimitingcollateraldamagetovitalorgans,suchasthelungs...

【 授权许可】

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