Immune cell recruitment to inflammatory loci is impaired in mice deficient in basement membrane protein laminin α4

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: inflammation;    leukocyte recruitment;   

DOI  :  10.1189/jlb.0110043

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Forleukocytestopenetratethevesselwall,theyneedtointeractsequentiallywiththeendothelialliningandtheperivascularBM.Thematrixproteinlaminin‐411isamajorconstituentofthevascularBM.Thelamininα4chainisacomponentoflaminin‐411andhasstructuralandsignalingfunctions.Here,weaddressedtheroleofBMlamininα4inleukocyterecruitmenttoinflammatoryloci.WeusedseveralrecruitmentmodelsinLam4−/−andWTmicetodeterminewhetherlackoflaminin‐411intheperivascularBMinfluencesextravasationofinflammatorycells.Recruitmentofallmajorleukocytesubsets(neutrophils,monocytes,andlymphocytes)wasreducedinLam4−/−micecomparedwithWT.Withtheuseofintravitalmicroscopy,weconcludedthatthisdecreasewasaresultofimpaireddiapedesisthroughthevesselwall,asneitherleukocyteadhesiontotheendothelialliningnormigrationinextravasculartissuewashamperedinLam4−/−mice.Collectively,ourdatasuggestareducedabilityofimmunecellstopenetratethevesselwallinmicedeficientinlamininα4...

【 授权许可】

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