Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
Galectin‐12 inhibits granulocytic differentiation of human NB4 promyelocytic leukemia cells while promoting lipogenesis | |
关键词: acute promyelocytic leukemia; lipid droplets; PPARγ; acute myeloid leukemia; myeloid differentiation; | |
DOI : 10.1189/jlb.1HI0316-134R | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
Asamemberofthegalectinfamilyofanimallectins,galectin‐12ispreferentiallyexpressedinadipocytesandleukocytes.Inadipocytes,galectin‐12isassociatedwithlipiddropletsandregulateslipidmetabolismandenergybalance,whereasitsroleinleukocytesisnotclear.Analysisofgalectin‐12expressioninapublicdatasetofacutemyeloidleukemia(AML)samplesrevealedthatitisselectivelyoverexpressedintheM3subtype,whichisalsoknownasacutepromyelocyticleukemia(APL).Toinvestigatetheroleofgalectin‐12inAPLcells,wemanipulateditsexpressionintheAPLcellline,NB4,andmeasuredresultanteffectsonall‐trans‐retinoicacid(ATRA)–inducedgranulocyticdifferentiation.Withadoxycycline‐induciblegeneknockdownsystem,wefoundthatsuppressionofgalectin‐12promotedATRA‐inducedneutrophildifferentiationbutinhibitedlipiddropletformation.Ourresultsindicatethatoverexpressionofgalectin‐12contributestoadifferentiationblockinAPLcells,andsuppressionofgalectin‐12facilitatesgranulocyticdifferentiation.Furthermore,thesedatasuggestthatlipogenesisandotheraspectsofmyeloiddifferentiationcanbedifferentiallyregulated.Takentogether,thesefindingssuggestthatgalectin‐12maybeatargetfortreatmentoftheATRA‐resistantsubsetofAPL...
【 授权许可】
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