Galectin‐12 inhibits granulocytic differentiation of human NB4 promyelocytic leukemia cells while promoting lipogenesis

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: acute promyelocytic leukemia;    lipid droplets;    PPARγ;    acute myeloid leukemia;    myeloid differentiation;   

DOI  :  10.1189/jlb.1HI0316-134R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Asamemberofthegalectinfamilyofanimallectins,galectin‐12ispreferentiallyexpressedinadipocytesandleukocytes.Inadipocytes,galectin‐12isassociatedwithlipiddropletsandregulateslipidmetabolismandenergybalance,whereasitsroleinleukocytesisnotclear.Analysisofgalectin‐12expressioninapublicdatasetofacutemyeloidleukemia(AML)samplesrevealedthatitisselectivelyoverexpressedintheM3subtype,whichisalsoknownasacutepromyelocyticleukemia(APL).Toinvestigatetheroleofgalectin‐12inAPLcells,wemanipulateditsexpressionintheAPLcellline,NB4,andmeasuredresultanteffectsonall‐trans‐retinoicacid(ATRA)–inducedgranulocyticdifferentiation.Withadoxycycline‐induciblegeneknockdownsystem,wefoundthatsuppressionofgalectin‐12promotedATRA‐inducedneutrophildifferentiationbutinhibitedlipiddropletformation.Ourresultsindicatethatoverexpressionofgalectin‐12contributestoadifferentiationblockinAPLcells,andsuppressionofgalectin‐12facilitatesgranulocyticdifferentiation.Furthermore,thesedatasuggestthatlipogenesisandotheraspectsofmyeloiddifferentiationcanbedifferentiallyregulated.Takentogether,thesefindingssuggestthatgalectin‐12maybeatargetfortreatmentoftheATRA‐resistantsubsetofAPL...

【 授权许可】

CC BY