Human neutrophil integrin α9β1: up‐regulation by cell activation and synergy with β2 integrins during adhesion to endothelium under flow

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: VCAM‐;    1;    endothelial cells;    rolling;   

DOI  :  10.1189/jlb.1009704

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Neutrophilβ1integrinexpressionandcontributiontocelladhesionwererevisitedinthisstudy.α9β1andα5β1appearedhereasthemainβ1integrinsexpressedonthemembraneofrestingplatelet‐depletedneutrophils—α6β1representing<15%andα2β1undetectable.Neutrophilactivationslightlyenhancedα5expression,didnotchangeα6,butresultedinatwo‐tothreefoldincreaseofα9β1,whichthenbecamethemajorβ1integrinoftheneutrophilmembrane.α9β1wastheonlyβ1integrintobeup‐regulatedaftertransendothelialmigrationacrossTNF‐α‐activatedHUVECs.Asα9β1bindsVCAM‐1,weanalyzeditsparticipationtoneutrophiladhesiontoTNF‐α‐activatedendothelialcells.Blockinganti‐α9mAbhadlittleeffectonneutrophilstaticadhesion,contrastingwiththestronginhibitionbyanti‐β2mAb.Underflowconditions,theanti‐α9mAbhadnoeffectbyitselfonneutrophiladhesiontoactivatedHUVECsbutenhancedtheblockingeffectofanti‐β2antibodiessignificantlyandfurtherenhancedthevelocityofβ2–blockedrollingneutrophils.Inconclusion,wedescribehereforthefirsttimeanearlyexclusiveup‐regulationofα9β1expressionamongallβ1integrinsduringneutrophilactivationandtransendothelialmigrationandapossiblyimportantsynergybetweenα9β1andβ2integrinsinstabilizingneutrophiladhesiontoendotheliumunderflowconditions...

【 授权许可】

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