期刊论文详细信息
Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
Technical Advance: Decreased helper T cells and increased natural killer cells in chronic periodontitis analyzed by a novel method for isolating resident lymphocytes | |
关键词: mononuclear cell isolation; explant culture; chemokine; chemotaxis; immunoregulation; | |
DOI : 10.1189/jlb.0212103 | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
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【 摘 要 】
DuringCP,thegingivalenvironmentisprimedtorecruitandactivatehominglymphocytes.However,detailedphenotypicandfunctionalcharacterizationofgingivaltissueresidentlymphocyteshasbeenchallengingasaresultoflimitationsassociatedwithavailableisolationmethodsandlimitedavailabilityofhumansamples.Thisstudyaimedtodevelopanovelexplantculturetechniqueforeffectivelyisolatinghumangingivallymphocytes.ThistechniquetakesadvantageofthenaturaltendencyofMNCstomigratetowardachemokinegradientgeneratedbythegingivalfibroblasts.TheexplantsystemallowedisolationofMNCswith∼95×higheryieldrelativetoconventionalapproaches.TheMNCyieldcorrelatesdirectlywithwetweightsofthetissues,andmaximalMNCsareisolatedduringthe2nddayoftheculture.TheexplantculturemethodandconventionalapproachproducesimilarMNCsubpopulationssuchasTh,Tc,andBcells.ChemokinesassociatedwithMNCmigrationbutnotcytokinesassociatedwithMNCproliferationanddifferentiationwereproducedintheexplantculture.Moreover,MNCmigrationinresponsetothesecretedchemokineswasinhibitedbyPTX.Tcellsdidnotundergoproliferationduringtheculture.However,theisolatedTcellsrespondedtomitogenicstimulationexvivo.Astatistically,significantlydecreasedThcellwithreducedCD25expressionalongwithincreasedNKandNKTcellsinCPareshown.However,thenumberofnaïveNKcellswasdecreasedsignificantlyinMNCs,suggestingactivationofNKcellsinCP...【 授权许可】
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