Technical Advance: Decreased helper T cells and increased natural killer cells in chronic periodontitis analyzed by a novel method for isolating resident lymphocytes

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: mononuclear cell isolation;    explant culture;    chemokine;    chemotaxis;    immunoregulation;   

DOI  :  10.1189/jlb.0212103

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

DuringCP,thegingivalenvironmentisprimedtorecruitandactivatehominglymphocytes.However,detailedphenotypicandfunctionalcharacterizationofgingivaltissueresidentlymphocyteshasbeenchallengingasaresultoflimitationsassociatedwithavailableisolationmethodsandlimitedavailabilityofhumansamples.Thisstudyaimedtodevelopanovelexplantculturetechniqueforeffectivelyisolatinghumangingivallymphocytes.ThistechniquetakesadvantageofthenaturaltendencyofMNCstomigratetowardachemokinegradientgeneratedbythegingivalfibroblasts.TheexplantsystemallowedisolationofMNCswith∼95×higheryieldrelativetoconventionalapproaches.TheMNCyieldcorrelatesdirectlywithwetweightsofthetissues,andmaximalMNCsareisolatedduringthe2nddayoftheculture.TheexplantculturemethodandconventionalapproachproducesimilarMNCsubpopulationssuchasTh,Tc,andBcells.ChemokinesassociatedwithMNCmigrationbutnotcytokinesassociatedwithMNCproliferationanddifferentiationwereproducedintheexplantculture.Moreover,MNCmigrationinresponsetothesecretedchemokineswasinhibitedbyPTX.Tcellsdidnotundergoproliferationduringtheculture.However,theisolatedTcellsrespondedtomitogenicstimulationexvivo.Astatistically,significantlydecreasedThcellwithreducedCD25expressionalongwithincreasedNKandNKTcellsinCPareshown.However,thenumberofnaïveNKcellswasdecreasedsignificantlyinMNCs,suggestingactivationofNKcellsinCP...

【 授权许可】

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