Human suppressive neutrophils CD16bright/CD62Ldim exhibit decreased adhesion

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: myeloid derived suppressor cells;    endotoxemia;    lipopolysaccharide;    chemotaxis;    adhesion/migration;   

DOI  :  10.1189/jlb.0612273

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Neutrophilsareessentialeffectorcellsinhostdefenseagainstinvadingpathogens.Regulationofadhesion,migration,andchemotacticprocessesisimportantinthehomingandactivationofthesecells.Werecentlydescribedthreedistinctsubsetsofcirculatinghumanneutrophilsinperipheralbloodduringacutesystemicinflammation.Onesubset,CD16bright/CD62Ldim,hasimmunesuppressivecharacteristicsbecauseitcaninhibitT‐cellproliferation.TheothertwosubsetsconsistofbandedCD16dim/CD62Lbrightandphenotypicallymature(normal)CD16bright/CD62Lbrightneutrophils.Thecurrentstudywasdesignedtodeterminetheadhesioncharacteristicsofthesedifferentneutrophilsubsets.AnalysisofadhesiontoactivatedendotheliumunderflowconditionsrevealedthatCD16bright/CD62LdimneutrophilsadheredlesscomparedwithCD16bright/CD62LbrightandCD16dim/CD62Lbrightneutrophils.ThisdecreaseinbindingcapacitycouldbemimickedintheotherneutrophilsubsetsbyblockingL‐selectin.ChemotaxisofCD16bright/CD62Ldimneutrophilstotheend‐targetchemoattractantN‐formylmethionine‐leucine‐phenylalaninewaslowercomparedwiththatfortheCD16dim/CD62Lbrightneutrophilsubset,whereaschemotaxistocell‐derivedchemoattractantCXCL8wascomparable.Ourdataindicatethatcaptureonendotheliumunderflowconditions,akeymechanismnecessaryforextravasation,ofCD16bright/CD62Ldimneutrophilstoinflammatorysitesisattenuated,whichmayfacilitatemigrationofthesecellstoothertissuelocalizations.Modulationofthisprocessisapotentialtargettomanipulateinflammationbecausepotentiationofthisimmunesuppressionmightaidinanti‐inflammatorytherapy...

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