Knock-Out of the Potassium Channel TASK-1 Leads to a Prolonged QT Interval and a Disturbed QRS Complex

Cellular Physiology and Biochemistry

关键词: Prolonged QT interval;    TASK-1 knock-out;    Cardiac repolarization;    In vivo electrophysiology;    Ventricular arrhythmias;   

DOI  :  10.1159/000331715

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background/Aims The aim of the study was to characterize the whole cell current of the two-pore domain potassium channel TASK-1 (K2P3) in mouse ventricular cardiomyocytes (I_TASK-1) and to analyze the cardiac phenotype of the TASK-1^-/- mice. Methods and Results We have quantified the ventricular I_TASK-1 current using the blocker A293 and TASK-1^-/- mice. Surface electrocardiogram recordings of TASK-1^-/- mice showed a prolonged QTc interval and a broadened QRS complex. The differences in electrocardiograms between wild type and TASK-1^-/- mice disappeared during sympathetic stimulation of the animals. Quantitative RT-PCR, patch clamp recordings and measurements of hemodynamic performance of TASK-1^-/- mice revealed no major compensatory changes in ion channel transcription. Action potential recordings of TASK-1^-/- mouse cardiomyocytes indicated that I_TASK-1 modulates action potential duration. Our in vivo electrophysiological studies showed that isoflurane, which activates TASK-1, slowed heart rate and atrioventricular conduction of wild-type but not of TASK-1^-/- mice. Conclusion The results of an invasive electrophysiological catheter protocol in combination with the observed QRS time prolongation in the surface electrocardiogram point towards a regulatory role of TASK-1 in the cardiac conduction system.

【 授权许可】

CC BY-NC-ND