Cellular Physiology and Biochemistry | |
The Hypertonic Environment Differentially Regulates Wild-type CFTR and TNR-CFTR Chloride Channels | |
关键词: CFTR; TNR-CFTR; Sodium chloride; Urea; Sucrose; MDCK cells; Hypertonic environment; Plasma membrane; | |
DOI : 10.1159/000322325 | |
学科分类:分子生物学,细胞生物学和基因 | |
来源: S Karger AG | |
【 摘 要 】
This study tested the hypotheses that the hypertonic environment of the renal medulla regulates the expression of cystic fibrosis transmembrane conductance regulator protein (CFTR) and its natural splice variant, TNR-CFTR. To accomplish this, Madin-Darby canine kidney (MDCK) stable cell lines expressing TNR-CFTR or CFTR were used. The cells were treated with hypertonic medium made with either NaCl or urea or sucrose (480 mOsm/kg or 560 mOsm/kg) to mimic the tonicity of the renal medulla environment. Western blot data showed that CFTR and TNR-CFTR total cell protein is increased by hypertonic medium, but using the surface biotinylation technique, only CFTR was found to be increased in cell plasma membrane. Confocal microscopy showed TNR-CFTR localization primarily at the endoplasmic reticulum and plasma membrane. In conclusion, CFTR and TNR-CFTR have different patterns of distribution in MDCK cells and they are modulated by a hypertonic environment, suggesting their physiological importance in renal medulla.
【 授权许可】
CC BY-NC-ND
【 预 览 】
Files | Size | Format | View |
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RO201901233374156ZK.pdf | 796KB | download |