Ubiquitin E3 Ligase A20 Contributes to Maintaining Epithelial Barrier Function

Cellular Physiology and Biochemistry

关键词: Epithelial barrier;    Allergen;    Ubiquitin E3 ligase;    Endosome;    Lysosome;   

DOI  :  10.1159/000341450

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background and Aims Epithelial barrier dysfunction is involved in the pathogenesis of allergic diseases; the mechanism is to be further understood. Ubiquitin E3 ligase A20 (A20) plays a role in maintaining the homeostasis in the body. This study aims to investigate the role of A20 in maintaining the epithelial barrier function. Methods Human intestinal epithelial cell line, Caco-2 cells, was cultured to monolayers to test the endocytosis and degradation of a model allergen, ovalbumin (OVA). The role of A20 in the endosome/lysosome fusion in epithelial cells was tested with A20-sufficient and A20-deficient Caco-2 cells and visualized by immunocytochemistry. Results Caco-2 cells could endocytose exogenous allergens (OVA) in culture. The endocytic OVA was degraded in A20-sufficient Caco-2 cells via the mechanism of endosome/lysosome fusion, while the A20-deficient Caco-2 monolayers converted the OVA to the basal compartment of transwells, which conserved the antigenicity reflected by that it induced T cell proliferation in an allergen-specific manner. A20 was required in the fusion of endosomes and lysosomes. Conclusion A20 contributes to maintaining the epithelial barrier function.

【 授权许可】

CC BY-NC-ND