Gene expression analysis of dendritic cells that prevent diabetes in NOD mice: analysis of chemokines and costimulatory molecules

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: diabetes;    T cells;    chemotaxis;    microarray analysis;   

DOI  :  10.1189/jlb.0311126

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

WehavedemonstratedpreviouslythatBM‐derivedDCscanpreventdiabetesdevelopmentandhaltprogressionofinsulitisinNODmice,themousemodeloftype1diabetes.TheDCpopulationthatwasmosteffectiveinthistherapyhadamaturephenotype,expressedhighlevelsofcostimulatorymolecules,andsecretedlowlevelsofIL‐12p70.TheprotectiveDCtherapyinducedTregandTh2cellsinvitroandinvivo.MicroarrayanalysisoftherapeuticandnontherapeuticDCpopulationsrevealeddifferencesintheexpressionofOX40L,CD200,Ym‐1,CCL2,andCCL5,whichcouldplayimportantrolesintheobservedDC‐mediatedtherapy.TheuniquepatternofcostimulatorymoleculesandchemokinesexpressedbythetherapeuticDCswasconfirmedbyflowcytometryandELISA.Usinganovelcell‐labelingand19FNMR,weobservedthatthechemokinessecretedbythetherapeuticDCsalteredthemigrationofdiabetogenicTh1cellsinvivoandattractedTh2cells.TheseresultssuggestthatthetherapeuticfunctionofDCsismediatedbyacombinationofcostimulatoryandchemokinepropertiesthatresultsintheattractionofdiabetogenicTh1andtheinductionofTh2and/orTregdifferentiation...

【 授权许可】

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