Real‐time in vivo imaging reveals the ability of neutrophils to remove Cryptococcus neoformans directly from the brain vasculature

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: phagocytes;    intravital;    fungus;    clearance;    complement;   

DOI  :  10.1189/jlb.4AB0715-281R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Althoughneutrophilsaretypicallythefirstimmunecellsattractedtoaninfectionsite,littleisknownabouthowneutrophilsdynamicallyinteractwithinvadingpathogensinvivo.Here,withtheuseofintravitalmicroscopy,wedemonstratethatneutrophilsmigratetothearrestedCryptococcusneoformans,aleadingagenttocausemeningoencephalitis,inthebrainmicrovasculature.FollowinginteractionswithC.neoformans,neutrophilswereseentointernalizetheorganismandthencirculatebackintothebloodstream,resultinginadirectremovaloftheorganismfromtheendothelialsurfacebeforeitstransmigrationintothebrainparenchyma.C.neoformansinfectionledtoenhancedexpressionofadhesionmoleculesmacrophage1antigenonneutrophilsandICAM‐1onbrainendothelialcells.Depletionofneutrophilsenhancedthebrainfungalburden.ComplementC3wascriticallyinvolvedintherecognitionofC.neoformansbyneutrophilsandsubsequentclearanceoftheorganismfromthebrain.Together,ourfindingofthedirectremovalofC.neoformansbyneutrophilsfromitsarrestedsitemayrepresentanovelmechanismofhostdefenseinthebrain,inadditiontotheknown,directkillingofmicroorganismsattheinfectionsites.Thesedataarethefirsttocharacterizedirectlythedynamicinteractionsofleukocyteswithamicrobeinthebrainofalivinganimal...

【 授权许可】

CC BY