Platelet activation and platelet–leukocyte interaction in generalized aggressive periodontitis

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: mean platelet volume;    calprotectin;    Porphyromonas gingivalis ;   

DOI  :  10.1189/jlb.4A1115-526RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Generalizedaggressiveperiodontitis(GAgP)isaninflammatorydiseaseofhostresponsetobacterialchallenge.Toexploretheroleofplateletsinhost–microbialinteractionsinpatientswithperiodontitis,124patientswithGAgPand57healthysubjectswereenrolled.Reliableindicatorsofsubclinicalplateletfunctionalstatus,plateletcount(PLT),plateletlargecellratio(PLCR),andmeanplateletvolume(MPV),weresignificantlylowerintheGAgPgroupthaninthecontrolgroupandwerenegativelycorrelatedwithclinicalperiodontalparameters.Thelevelsofimportantcytosolicproteininneutrophils,calprotectin(S100A8/A9)inplasma,andgingivalcrevicularfluid(GCF)weresignificantlyhigherinpatientswithGAgPcomparedwithhealthysubjects.Moreover,theGCFcalprotectinlevelwasnegativelycorrelatedwithPLCRandMPVvalues.Toexplorethepossiblemechanismsofchangesinplateletindicesinperiodontitis,flowcytometryanalysiswasperformed,andpatientswithGAgPwerefoundtohaveahigherstatusofplateletactivationcomparedwithhealthycontrols.Porphyromonasgingivalis(P.gingivalis)andrecombinanthumanS100A8/A9(rhS100A8/A9)inducedplateletactivationandfacilitatedplatelet–leukocyteaggregateformationinwholebloodofhealthysubjects.InresponsetoP.gingivalisandrhS100A8/A9,plateletsfrompatientswithGAgPincreasedactivationandincreasedformationofplatelet–leukocyteaggregatescomparedwiththosefromhealthysubjects.PlateletaggregatesandplateletsattachedtoleukocyteswerefoundongingivaltissuesfrompatientswithGAgP,suggestingthatdecreasedplateletsizeandcountinthecirculationmightberelatedtoconsumptionoflarge,activatedplateletsatinflamedgingiva.Plateletsmayhaveapreviouslyunrecognizedroleinhostresponsetoperiodontalinfection...

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