| Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
| Tumor necrosis factor α‐converting enzyme (TACE/ADAM17) mediates ectodomain shedding of the scavenger receptor CD163 | |
| 关键词: endocytosis; hemoglobin; pathogenesis; sepsis; macrophage; | |
| DOI : 10.1189/jlb.0410235 | |
| 学科分类：生理学 | |
| 来源: Federation of American Societies for Experimental Biology | |
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【 摘 要 】
CD163isexpressedspecificallyinthemonocyte/macrophagelineage,whereitmediatesuptakeofhaptoglobin‐hemoglobincomplexes,leadingtometabolismoftheoxidativehememolecule.SheddingoftheCD163ectodomainfromthecellsurfaceproducesasCD163plasmaprotein,andapositivecorrelationisseenbetweenthesCD163plasmalevelandtheseverityofvariousinfectiousandinflammatorydiseases.Inthepresentanalysisofthephorbolester‐inducedsheddingofsCD163inCD163cDNA‐transfectedHEK293cells,weusedmetalloproteinaseinhibitorsandsiRNA‐mediatedinhibitionofmetalloproteinasestoidentifyTACE/ADAM17asanenzymeresponsibleforPMA‐inducedcleavageofthemembrane‐proximalregionofCD163.AsTACE/ADAM17‐mediatedsheddingofTNF‐αisup‐regulatedinmacrophagessubjectedtoinflammatorystimuli,thepresentresultsnowprovidealikelyexplanationforthestrongempiricalrelationshipbetweenthesCD163plasmalevelandinfectious/inflammatorydiseasesrelatingtomacrophageactivity...
【 授权许可】
CC BY
【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201901231021927ZK.pdf | 890KB |  download |
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