Technical Advance: Fluorescent reporter reveals insights into eomesodermin biology in cytotoxic lymphocytes

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: CD8 T cells;    natural killer cells;    memory;    homeostasis;    differentiation;   

DOI  :  10.1189/jlb.0812400

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

TheT‐boxtranscriptionfactorEomesisexpressedincytotoxicimmunecellsandplaysanimportantroleindevelopment,maintenance,andfunctionofthesecelltypes.IdentificationandseparationofcellswithdifferentialEomesexpressionwouldallowforbetterunderstandingofthetranscriptionalprogramgoverningthesecytotoxiclymphocytes.Here,wereporttheuseofanEomesgfp‐targetedmouseallelethatdisplaysrobustfidelitytoEomesproteinexpressioninNKandTcells.UseofthisreportermouserevealedthatEomesexpressioninantiviraleffectorcellsdidnotcorrelatewithenhancedcytotoxicitybutratherwasassociatedwithmoreefficientcentralmemorydifferentiation.WeakeningofreporteractivityinEomes‐deficientCD8+TcellsrevealedacriticalroleforEomesproteininmaintainingcentralmemorycellsthathaveactivatedtheEomeslocus.CharacterizationofreporteractivityinNKlineagecellsalsopermittedidentificationofanovelintermediateofNKcellmaturation.Thus,themurineEomesgfp‐targetedalleleprovidesanovelopportunitytoexploreEomesbiologyincytotoxiclymphocytes...

【 授权许可】

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