Chemometric analysis of attenuated total reflectance infrared spectra of Proteus mirabilis strains with defined structures of LPS:

Innate Immunity

PaulinaZarnowiec¹ 

关键词: Differentiation index;    hierarchical cluster analysis;    infrared spectroscopy;    Kohonen network;    lipopolysaccharide;    principal component analysis;    Proteus spp;    r;    om forest;   

DOI  :  10.1177/1753425916647470

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Proteus spp. strains are some of the most important pathogens associated with complicated urinary tract infections and bacteremia affecting patients with immunodeficiency and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods have been developed for this pathogen. However, these methods are labor intensive and time consuming. We evaluated a new method of differentiation between strains. A collection of Proteus spp. strains was analyzed by attenuated total reflectance Fourier transform infrared (ATR FT-IR) spectroscopy in the mid-infrared region. ATR FT-IR spectroscopy used in conjunction with a diamond ATR accessory directly produced the biochemical profile of the surface chemistry of bacteria. We conclude that a combination of ATR FT-IR spectroscopy and mathematical modeling provides a fast and reliable alternative for discrimination between Proteus isolates, contributing to epidemiological research.

【 授权许可】

CC BY