Altered Levels of Histone Deacetylase OsHDT1 Affect Differential Gene Expression Patterns in Hybrid Rice

PLoS One

Chen Li¹  Caiguo Xu¹  Limin Huang¹  Dao-Xiu Zhou¹  Yu Zhao¹ 

[1]National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China

关键词: Gene expression;    Rice;    RNA interference;    Histones;    Gene regulation;    Metabolic pathways;    Genetically modified plants;    Xenobiotic metabolism;   

DOI  :  10.1371/journal.pone.0021789

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Hybrids between different inbred varieties display novel patterns of gene expression resulted from parental variation in allelic nucleotide sequences. To study the function of chromatin regulators in hybrid gene expression, the histone deacetylase gene OsHDT1 whose expression displayed a circadian rhythm was over-expressed or inactivated by RNAi in an elite rice parent. Increased OsHDT1 expression did not affect plant growth in the parent but led to early flowering in the hybrid. Nonadditive up-regulation of key flowering time genes was found to be related to flowering time of the hybrid. Over-expression of OsHDT1 repressed the nonadditive expression of the key flowering repressors in the hybrid (i.e. OsGI and Hd1) inducing early flowering. Analysis of histone acetylation suggested that OsHDT1 over-expression might promote deacetylation on OsGI and Hd1 chromatin during the peak expression phase. High throughput differential gene expression analysis revealed that altered OsHDT1 levels affected nonadditive expression of many genes in the hybrid. These data demonstrate that nonadditive gene expression was involved in flowering time control in the hybrid rice and that OsHDT1 level was important for nonadditive or differential expression of many genes including the flowering time genes, suggesting that OsHDT1 may be involved in epigenetic control of parental genome interaction for differential gene expression.

【 授权许可】

CC BY