Up-regulation of integrin expression in lung adenocarcinoma cells caused by bacterial infection: in vitro study:

Innate Immunity

SeanGravelle¹ 

关键词: inflammation;    integrin linked kinase;    Pseudomonas aeruginosa;    epithelial cell receptors;   

DOI  :  10.1177/1753425909106170

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Integrins are a large family of adhesion receptors that are known to be key signaling molecules in both physiological and pathological processes. Previous studies have demonstrated that the expression of integrin receptors in the pulmonary epithelium can change under various pathological conditions, such as injury, inflammation, or malignant transformation. We hypothesize that integrin expression can be altered by stimulation of lung epithelial cells with an opportunistic bacterial pathogen Pseudomonas aeruginosa. Using the A549 adenocarcinoma cell line that expressed a low level of several integrin subunits we have demonstrated that P. aeruginosa infection in vitro caused a rapid up-regulation of α5, αv, β1, and β4 integrins at both the mRNA and protein level. Neither heat-killed P. aeruginosa strain PAK nor its live isogenic mutants lacking pili or lipopolysaccharide (LPS) core oligosaccharide showed any effect on integrin expression in A549 cells as compared to the use of the wild-type PAK strain. Thes...

【 授权许可】

CC BY