Differential effect of molecular mass hyaluronan on lipopolysaccharide-induced damage in chondrocytes:

Innate Immunity

Giuseppe M.Campo¹ 

关键词: hyaluronan;    chondrocytes;    LPS;    NF-κB;    nitric oxide;    inflammatory cytokines;    apoptosis;   

DOI  :  10.1177/1753425909340419

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Hyaluronan is a biological polysaccharide that may exist in different degrees of polymerization. Several investigations reported that low molecular mass hyaluronan may have pro-inflammatory activity, while high molecular mass hyaluronan can exert beneficial effects. Starting from these data, the aim of this study was to investigate the effect of hyaluronan of different molecular mass in mouse articular chondrocyte cultures stimulated with lipopolysaccharide (LPS). Inflammation was induced in chondrocytes by acute treatment with 2.0 µg/ml LPS. High levels of tumour necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, interferon (IFN)-γ and iNOS gene expression and their related proteins were found in chondrocytes 24 h after treatment with LPS. High concentrations of NO, NF-κB activation, IκBα phosphorylation and apoptosis, evaluated by the increase in caspase-3 expression and its related protein amount were also produced by LPS stimulation. In contrast, LPS reduced aggrecan and collagen type II (Col2A) expr...

【 授权许可】

CC BY