Quantitative analysis of benzodiazepines in vitreous humor by high-performance liquid chromatography:

SAGE Open Medicine

ElhamBazmi¹ 

关键词: Benzodiazepines;    vitreous humor;    liquid–liquid extraction;    high-performance liquid chromatography;    method validation;    forensic toxicology;   

DOI  :  10.1177/2050312116666243

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Objective:Benzodiazepines are frequently screened drugs in emergency toxicology, drugs of abuse testing, and in forensic cases. As the variations of benzodiazepines concentrations in biological samples during bleeding, postmortem changes, and redistribution could be biasing forensic medicine examinations, hence selecting a suitable sample and a validated accurate method is essential for the quantitative analysis of these main drug categories. The aim of this study was to develop a valid method for the determination of four benzodiazepines (flurazepam, lorazepam, alprazolam, and diazepam) in vitreous humor using liquid–liquid extraction and high-performance liquid chromatography.Methods:Sample preparation was carried out using liquid–liquid extraction with n-hexane: ethyl acetate and subsequent detection by high-performance liquid chromatography method coupled to diode array detector. This method was applied to quantify benzodiazepines in 21 authentic vitreous humor samples. Linear curve for each drug was ...

【 授权许可】

CC BY