Activation of indoleamine 2,3-dioxygenase by LPS in a porcine model:

Innate Immunity

ElisaWirthgen¹ 

关键词: Indoleamine 2;    3-dioxygenase;    kynurenine pathway;    LPS;    pig;   

DOI  :  10.1177/1753425913481252

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Indoleamine 2,3-dioxygenase (IDO) is a rate-limiting enzyme for the degradation of tryptophan (Trp) along the kynurenine (Kyn) pathway, and its increased activation is associated with immunologic disorders. Because the specific role of IDO activation is not yet completely clear, the aim of the present study was to establish a pig model of IDO activation for further research. The activation of IDO in pigs was induced experimentally by LPS stimulation in vivo and ex vivo. IDO activation was characterized by measuring Trp, Trp metabolites and IDO protein expression in blood, liver, lung, muscle and different brain areas. The results show that the in vivo LPS administration induced increased plasma concentrations of TNF-α and IL-10, a depletion of Trp and an increase of Kyn, indicating an elevated enzymatic activity of IDO. This was supported by an LPS-induced IDO protein expression in blood, liver and lung. The ex vivo LPS stimulation also resulted in increased TNF-α concentrations and an IDO activation, cha...

【 授权许可】

CC BY