External induction of heat shock stimulates the immune response and longevity of Caenorhabditis elegans towards pathogen exposure:

Innate Immunity

UdayakumarPrithika¹ 

关键词: C. elegans;    heat shock factor;    heat shock proteins;    insulin signaling pathway;    longevity;    pathogen resistance;   

DOI  :  10.1177/1753425916654557

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Heat shock proteins (HSPs) are highly chaperonic molecules that give immediate response during any stress, tissue damage or bacterial infections. In the present study, the role of HSPs upon bacterial encounter is studied by applying external heat induction to live Caenorhabditis elegans. Heat shock was observed to increase the life span of wild type C. elegans upon pathogenic encounter, indicating a role of HSPs in bacterial infection and immunity. Similar increase in resistance towards pathogenesis observed in long-lived C. elegans daf-2 mutants and the increase in the lifespan indicated a role for the insulin/IGF-1 signaling (IIS) pathway in HSP-mediated pathogenic resistance. The microscopic observation of C. elegans after external heat induction and sequential exposure of pathogens indicated reduction of egg viability. Results of Real-time PCR and immunoblotting analysis of candidate genes revealed that heat shock and IIS pathways collaborate in the observed pathogenic resistance and further suggested...

【 授权许可】

CC BY