Sera from patients with Crohn’s disease break bacterial lipopolysaccharide tolerance of human intestinal epithelial cells via MD-2 activity:

Innate Immunity

PhilippeSeksik¹ 

关键词: inflammatory bowel disease;    intestinal epithelial cells;    lipopolysaccharide tolerance;    MD-2;   

DOI  :  10.1177/1753425909357076

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Myeloid differentiation (MD)-2 is linked to the cell surface as a Toll-like receptor (TLR) 4-bound protein though may also function as a soluble receptor to enable the lipopolysaccharide (LPS)-driven response. We recently demonstrated the importance of MD-2 either as a cell-associated or as a soluble receptor in the control of intestinal epithelial cell response toward LPS. High levels of circulating MD-2 were recently proposed as a risk factor for infectious/ inflammatory diseases as septic shock. We hypothesized that MD-2 might be present in sera from patients with inflammatory bowel disease and have pathogenic consequences. We analysed MD-2 activity in sera from patients with inflammatory bowel disease or from healthy subjects. We measured MD-2 activity as the capacity to mediate LPS-driven stimulation of intestinal epithelial cells (HT29). We found that sera from patients with inflammatory bowel disease, particularly Crohn’s disease, endowed HT29 cells with a markedly higher LPS-dependent stimulating ...

【 授权许可】

CC BY