Age-dependent cellular reactions of the human immune system of humanized NOD scid gamma mice on LPS stimulus:

Innate Immunity

AnjaRodewohl¹ 

关键词: Humanized mice;    PTX3;    sepsis;    sepsis mouse model;   

DOI  :  10.1177/1753425917690814

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Despite sepsis being a life-threatening disease, targeted drugs that improve the therapy of affected patients are still lacking. Infants and adults differ in the maturity level of their immune system and this results in distinct reactions to Gram-negative bacteria. To study reactions of human immune cells in vivo, we used NOD scid gamma mice transplanted with human CD34+ stem cells to engraft a functional human immune system. Human cells undergo differentiation and maturation in these mice after transplantation and, accordingly, animals were divided into two groups: 8–13 wk and 15–22 wk after transplantation. Endotoxemia was induced by injecting LPS. Six h later, mice were euthanized. In both groups, LPS stimulation induced a decrease of CD14+ monocytes in peripheral blood, an up-regulation of activation markers on different cell subsets such as myeloid dendritic cells, and a release of the human cytokines TNF-α, IL-6 and IL-10. However, significant differences were detected with regard to the amounts of ...

【 授权许可】

CC BY