Cortisol is a potent modulator of lipopolysaccharide-induced interferon signaling in macrophages:

Innate Immunity

Anja M.Billing¹ 

关键词: 2-D DIGE;    cortisol;    MX1;    FKBP51;    IRF3;    LPS;   

DOI  :  10.1177/1753425910369269

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

The effects of cortisol (CORT) on resting and lipopolysaccharide (LPS)-activated monocyte-derived THP-1 macrophages were investigated by proteomics. Forty-seven proteins were found to be modulated, 20 by CORT, 11 by LPS, and 16 by CORT and LPS. Cortisol-sensitive chaperones and cytoskeletal proteins were mostly repressed. HCLS1, MGN, and MX1 were new proteins identified to be under the transcriptional control of this steroid and new CORT-sensitive variants of MX1, SYWC and IFIT3 were found. FKBP51, a known CORT target gene, showed the strongest response to CORT and synergism with LPS. In resting THP-1 macrophages, 18 proteins were modulated by CORT, with 15 being down-regulated. Activation of macrophages by LPS was associated with enhanced expression of immune response and metabolic proteins. In activated macrophages, CORT had a more equilibrated effect and almost all metabolism-related proteins were up-regulated, whereas immune response proteins were mostly down-regulated. The majority of the LPS up-regu...

【 授权许可】

CC BY