Combined loss of cRel/p50 subunits of NF-κB leads to impaired innate host response in sepsis:

Innate Immunity

EmilieCourtine¹ 

关键词: NF-κB;    sepsis;    inflammation;    mice;    expression profiles;   

DOI  :  10.1177/1753425912440296

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

NF-κB, which comprises homo- and hetero-dimers of the five members of the Rel family, plays a crucial role in immunity to infection. The cRel and p50 subunits have been implicated in the development and function of the immune cells, but their in vivo importance remains poorly explored in sepsis. We aimed to study the impact of the combined loss of these two subunits on the innate response to infection in a cecal ligation and puncture model of sepsis. We have explored the possible defects in host defense, including pathogen clearance, bacterial phagocytosis and cytokine plasma release. We also performed gene profiling of cRel−/−p50−/− and wild-type LPS-stimulated peritoneal macrophages. Deficiency of cRel and p50 led to enhanced mortality to sepsis that was associated with defective macrophages phagocytosis, decreased bacterial clearance and moderate cytokine response. Transcription profile analysis revealed a common inflammatory response but a significant down-regulated transcription of genes encoding for...

【 授权许可】

CC BY