Early transcriptional events in the udder and teat after intra-mammary Escherichia coli and Staphylococcus aureus challenge:

Innate Immunity

WolframPetzl¹ 

关键词: Cow;    mastitis;    subclinical;    host-response;    model;   

DOI  :  10.1177/1753425916640057

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Intra-mammary bacterial infections can result in harmful clinical mastitis or subclinical mastitis with persistent infections. Research during the last decades closely examined the pathophysiology of inflamed udders. Initial events after pathogen perception but before the onset of mastitis have not been examined in vivo. The objective of this study was to develop a mastitis model in cows by monitoring initial transcriptional pathogen-specific host response before clinical signs occur. We applied a short-term infection model to analyse transcripts encoding chemokines, cytokines and antimicrobial molecules in the teat cistern (TC) and lobulo-alveolar parenchyma (LP) up to 3 h after challenge with E. and Staphylococcus aureus. Both pathogens elicited an immune reaction by 1 h after challenge. Escherichia coli induced all analysed factors (CCL20, CXCL8, TNF, IL6, IL12B, IL10, LAP, S100A9); however, S. aureus failed to induce IL12B, IL10, LAP and S100A9 expression. The E. coli-induced up-regulation was 25–105 ...

【 授权许可】

CC BY