Lipopolysaccharide pretreatment of the udder protects against experimental Escherichia coli mastitis:

Innate Immunity

WolframPetzl¹ 

关键词: Cattle;    E. coli;    endotoxin tolerance;    LPS;    mastitis;   

DOI  :  10.1177/1753425911422407

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Exposure to pathogen-associated molecular patterns such as LPS can cause an immune refractory state in mammals known as endotoxin tolerance (ET), resulting in a decreased inflammatory response after pathogen contact. This ET concept was used to reduce the severity of an experimentally-induced clinical mastitis. Cows were pretreated with 1 µg LPS per udder quarter and challenged 72 h (group L72EC) or 240 h (group L240EC) later with 500 CFU Escherichia coli. Pretreated animals showed no leukopenia after challenge, no (L72EC), or only slightly (L240EC), elevated body temperature and significantly reduced systemic and local clinical scores compared with cows that were not pretreated. Whereas an increase of milk somatic cell count after the E. coli challenge was abrogated in L72EC animals, it was significantly delayed in the L240EC group. In both pretreated groups the bacterial load in milk was markedly reduced. Based on the expression of inflammation-related genes in lobulo-alveolar mammary tissue, the toleri...

【 授权许可】

CC BY