Sendai virus C protein limits NO production in infected RAW264.7 macrophages:

Innate Immunity

ErdenezayaOdkhuu¹ 

关键词: Accessory proteins;    double-str;    ed RNA;    macrophages;    NO;    paramyxovirus;   

DOI  :  10.1177/1753425918796619

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

To suppress virus multiplication, infected macrophages produce NO. However, it remains unclear how infecting viruses then overcome NO challenge. In the present study, we report the effects of accessory protein C from Sendai virus (SeV), a prototypical paramyxovirus, on NO output. We found that in RAW264.7 murine macrophages, a mutant SeV without C protein (4C(–)) significantly enhanced inducible NO synthase (iNOS) expression and subsequent NO production compared to wild type SeV (wtSeV). SeV 4C(-) infection caused marked production of IFN-β, which is involved in induction of iNOS expression via the JAK-STAT pathway. Addition of anti-IFN-β Ab, however, resulted in only marginal suppression of NO production. In contrast, NF-κB, a primarily important factor for transcription of the iNOS gene, was also activated by 4C(–) infection but not wtSeV infection. Induction of NO production and iNOS expression by 4C(–) was significantly suppressed in cells constitutively expressing influenza virus NS1 protein that can...

【 授权许可】

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